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cd80  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cd80
    Cd80, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd80/product/Cell Signaling Technology Inc
    Average 94 stars, based on 31 article reviews
    cd80 - by Bioz Stars, 2026-03
    94/100 stars

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    a Schematic illustration of scRNA-seq analysis of the CT26-luc syngeneic mouse model. b Proportion of tumor-infiltrating immune cells labeled via scRNA-seq. c UMAP plot of scRNA-seq profiles from macrophages within the tumor. d Counts of clustered macrophages in groups. e UMAP plot of relative expression for the indicated gene ( Trem2 , Nos2 , <t>Cd80</t> , Cd274 ). f Flow cytometry analysis of macrophage PD-L1 and H2-Kd expression in groups ( g ). Mean fluorescence intensity (MFI) calculated from flow cytometry profiles of macrophage PD-L1 and H2-Kd. Data are presented as mean ± SD ( n = 3 technical replicates). h Heatmap of MHC I and MHC II encoded gene expression in BMDMs after various CAR signal transduction. i Normalized MFI of macrophage PD-L1 and H2-Kd with NF-κBi (PDTC, 100 μM). Data are presented as mean ± SD (from n = 3 technical replicates). j Schematic representation of CAR signal transduction leading to upregulated PD-L1 and MHC I expression. k Recorded BLI of the CT26-luc syngeneic mouse model treated with UTD, PD-L1 siRNA, CAR-M, and combination therapy ( n = 4 mice). l Counts of clustered T cells in groups. m Schema of the mechanism of potent synergy between tailored CAR-Ms and ICB. Source data are provided as a Source Data file.
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    a Schematic illustration of scRNA-seq analysis of the CT26-luc syngeneic mouse model. b Proportion of tumor-infiltrating immune cells labeled via scRNA-seq. c UMAP plot of scRNA-seq profiles from macrophages within the tumor. d Counts of clustered macrophages in groups. e UMAP plot of relative expression for the indicated gene ( Trem2 , Nos2 , <t>Cd80</t> , Cd274 ). f Flow cytometry analysis of macrophage PD-L1 and H2-Kd expression in groups ( g ). Mean fluorescence intensity (MFI) calculated from flow cytometry profiles of macrophage PD-L1 and H2-Kd. Data are presented as mean ± SD ( n = 3 technical replicates). h Heatmap of MHC I and MHC II encoded gene expression in BMDMs after various CAR signal transduction. i Normalized MFI of macrophage PD-L1 and H2-Kd with NF-κBi (PDTC, 100 μM). Data are presented as mean ± SD (from n = 3 technical replicates). j Schematic representation of CAR signal transduction leading to upregulated PD-L1 and MHC I expression. k Recorded BLI of the CT26-luc syngeneic mouse model treated with UTD, PD-L1 siRNA, CAR-M, and combination therapy ( n = 4 mice). l Counts of clustered T cells in groups. m Schema of the mechanism of potent synergy between tailored CAR-Ms and ICB. Source data are provided as a Source Data file.
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    a Schematic illustration of scRNA-seq analysis of the CT26-luc syngeneic mouse model. b Proportion of tumor-infiltrating immune cells labeled via scRNA-seq. c UMAP plot of scRNA-seq profiles from macrophages within the tumor. d Counts of clustered macrophages in groups. e UMAP plot of relative expression for the indicated gene ( Trem2 , Nos2 , <t>Cd80</t> , Cd274 ). f Flow cytometry analysis of macrophage PD-L1 and H2-Kd expression in groups ( g ). Mean fluorescence intensity (MFI) calculated from flow cytometry profiles of macrophage PD-L1 and H2-Kd. Data are presented as mean ± SD ( n = 3 technical replicates). h Heatmap of MHC I and MHC II encoded gene expression in BMDMs after various CAR signal transduction. i Normalized MFI of macrophage PD-L1 and H2-Kd with NF-κBi (PDTC, 100 μM). Data are presented as mean ± SD (from n = 3 technical replicates). j Schematic representation of CAR signal transduction leading to upregulated PD-L1 and MHC I expression. k Recorded BLI of the CT26-luc syngeneic mouse model treated with UTD, PD-L1 siRNA, CAR-M, and combination therapy ( n = 4 mice). l Counts of clustered T cells in groups. m Schema of the mechanism of potent synergy between tailored CAR-Ms and ICB. Source data are provided as a Source Data file.
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    a Schematic illustration of scRNA-seq analysis of the CT26-luc syngeneic mouse model. b Proportion of tumor-infiltrating immune cells labeled via scRNA-seq. c UMAP plot of scRNA-seq profiles from macrophages within the tumor. d Counts of clustered macrophages in groups. e UMAP plot of relative expression for the indicated gene ( Trem2 , Nos2 , <t>Cd80</t> , Cd274 ). f Flow cytometry analysis of macrophage PD-L1 and H2-Kd expression in groups ( g ). Mean fluorescence intensity (MFI) calculated from flow cytometry profiles of macrophage PD-L1 and H2-Kd. Data are presented as mean ± SD ( n = 3 technical replicates). h Heatmap of MHC I and MHC II encoded gene expression in BMDMs after various CAR signal transduction. i Normalized MFI of macrophage PD-L1 and H2-Kd with NF-κBi (PDTC, 100 μM). Data are presented as mean ± SD (from n = 3 technical replicates). j Schematic representation of CAR signal transduction leading to upregulated PD-L1 and MHC I expression. k Recorded BLI of the CT26-luc syngeneic mouse model treated with UTD, PD-L1 siRNA, CAR-M, and combination therapy ( n = 4 mice). l Counts of clustered T cells in groups. m Schema of the mechanism of potent synergy between tailored CAR-Ms and ICB. Source data are provided as a Source Data file.
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    a Schematic illustration of scRNA-seq analysis of the CT26-luc syngeneic mouse model. b Proportion of tumor-infiltrating immune cells labeled via scRNA-seq. c UMAP plot of scRNA-seq profiles from macrophages within the tumor. d Counts of clustered macrophages in groups. e UMAP plot of relative expression for the indicated gene ( Trem2 , Nos2 , <t>Cd80</t> , Cd274 ). f Flow cytometry analysis of macrophage PD-L1 and H2-Kd expression in groups ( g ). Mean fluorescence intensity (MFI) calculated from flow cytometry profiles of macrophage PD-L1 and H2-Kd. Data are presented as mean ± SD ( n = 3 technical replicates). h Heatmap of MHC I and MHC II encoded gene expression in BMDMs after various CAR signal transduction. i Normalized MFI of macrophage PD-L1 and H2-Kd with NF-κBi (PDTC, 100 μM). Data are presented as mean ± SD (from n = 3 technical replicates). j Schematic representation of CAR signal transduction leading to upregulated PD-L1 and MHC I expression. k Recorded BLI of the CT26-luc syngeneic mouse model treated with UTD, PD-L1 siRNA, CAR-M, and combination therapy ( n = 4 mice). l Counts of clustered T cells in groups. m Schema of the mechanism of potent synergy between tailored CAR-Ms and ICB. Source data are provided as a Source Data file.
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    a Schematic illustration of scRNA-seq analysis of the CT26-luc syngeneic mouse model. b Proportion of tumor-infiltrating immune cells labeled via scRNA-seq. c UMAP plot of scRNA-seq profiles from macrophages within the tumor. d Counts of clustered macrophages in groups. e UMAP plot of relative expression for the indicated gene ( Trem2 , Nos2 , Cd80 , Cd274 ). f Flow cytometry analysis of macrophage PD-L1 and H2-Kd expression in groups ( g ). Mean fluorescence intensity (MFI) calculated from flow cytometry profiles of macrophage PD-L1 and H2-Kd. Data are presented as mean ± SD ( n = 3 technical replicates). h Heatmap of MHC I and MHC II encoded gene expression in BMDMs after various CAR signal transduction. i Normalized MFI of macrophage PD-L1 and H2-Kd with NF-κBi (PDTC, 100 μM). Data are presented as mean ± SD (from n = 3 technical replicates). j Schematic representation of CAR signal transduction leading to upregulated PD-L1 and MHC I expression. k Recorded BLI of the CT26-luc syngeneic mouse model treated with UTD, PD-L1 siRNA, CAR-M, and combination therapy ( n = 4 mice). l Counts of clustered T cells in groups. m Schema of the mechanism of potent synergy between tailored CAR-Ms and ICB. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Intraperitoneal programming of tailored CAR macrophages via mRNA lipid nanoparticle to boost cancer immunotherapy

    doi: 10.1038/s41467-025-67674-9

    Figure Lengend Snippet: a Schematic illustration of scRNA-seq analysis of the CT26-luc syngeneic mouse model. b Proportion of tumor-infiltrating immune cells labeled via scRNA-seq. c UMAP plot of scRNA-seq profiles from macrophages within the tumor. d Counts of clustered macrophages in groups. e UMAP plot of relative expression for the indicated gene ( Trem2 , Nos2 , Cd80 , Cd274 ). f Flow cytometry analysis of macrophage PD-L1 and H2-Kd expression in groups ( g ). Mean fluorescence intensity (MFI) calculated from flow cytometry profiles of macrophage PD-L1 and H2-Kd. Data are presented as mean ± SD ( n = 3 technical replicates). h Heatmap of MHC I and MHC II encoded gene expression in BMDMs after various CAR signal transduction. i Normalized MFI of macrophage PD-L1 and H2-Kd with NF-κBi (PDTC, 100 μM). Data are presented as mean ± SD (from n = 3 technical replicates). j Schematic representation of CAR signal transduction leading to upregulated PD-L1 and MHC I expression. k Recorded BLI of the CT26-luc syngeneic mouse model treated with UTD, PD-L1 siRNA, CAR-M, and combination therapy ( n = 4 mice). l Counts of clustered T cells in groups. m Schema of the mechanism of potent synergy between tailored CAR-Ms and ICB. Source data are provided as a Source Data file.

    Article Snippet: Primary antibodies used in the study are listed as follows: F4/80 (123117, BioLegend), F4/80 (111604, BioLegend), CD80 (50446-R014-A-25, Sino Biological), CD206 (FAB2535P, R&D), CD206 (#24595, CST), CD3 (100228, BD), CD3e (561108, BD), CD4 (550954, BD), CD8 (K0227-A64, MBL), CD25 (564021, BD), CD44 (103025, BioLegend), CD62L (104405, BioLegend), IFNr (505807, BioLegend), and goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077, abcam).

    Techniques: Labeling, Expressing, Flow Cytometry, Fluorescence, Gene Expression, Transduction